Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase

نویسندگان

A Jafarian

D Abedi

MR Fazeli

چکیده مقاله:

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compared with PGA-450, this immobilized enzyme showed a high synthesis activity. The optimum conditions for synthetic activity was at pH 6, 25°C and 2:6 (6-APA:PGME) substrate ratio.

Download for Free

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

optimization of enzymatic synthesis of ampicillin using cross-linked aggregates of penicillin g acylase

penicillin g acylase from e. coli ta1 was immobilized by cross-linked enzyme aggregates (clea), a new method for immobilization. this biocatalyst and commercial immobilized penicillin g acylase (pga-450) were used to study the effect of ph, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (pgme) and 6-aminopenicillanic acid (6-apa). compare...

متن کامل

Improving Selectivity and Productivity of the Enzymatic Synthesis of Ampicillin with Immobilized Penicillin G Acylase

An experimental design was applied to improve the reaction conditions for enzymatic synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA), catalyzed by penicillin G acylase from E. coli immobilized on an agarose-glyoxyl derivative. The presence and magnitude of interactions between reaction variables were estimated using a 2 factorial design. A batc...

متن کامل

Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase

The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous medium was investigated. The parameters studied were: time course of amoxicillin production, concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6APA) and the e ect of enzyme (PGA) content and pH, under variable and constant conditions and temperature variations. In...

متن کامل

Improving the diastereoselectivity of penicillin G acylase for ampicillin synthesis from racemic substrates.

Semi-synthetic β-lactam antibiotics are synthesized enzymatically with the use of penicillin G acylase (PGA). Currently, PGA only exhibits weak diastereoselectivity with respect to the alpha amino group of rac-phenylglycine methyl ester (rac-PGME) when it is coupled with 6-aminopenicillanic acid to synthesize ampicillin. Therefore, we sought to improve the diastereoselectivity of PGA by targeti...

متن کامل